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Nanjing Pars Biochem CO.,Ltd

Products >> Aflatoxin M1 (AFM1) ELISA Test Kit

Aflatoxin M1 (AFM1) ELISA Test Kit

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Description
Product Name: Aflatoxin M1 (AFM1) ELISA Test Kit
Supply Ability: 500000kits/year
Related proudcts elisa test kit, aflatoxin m1, food test kit,
Specifications 96T
Price Term: CNF
Port of loading: NANJING
Minimum Order 1box
Unit Price:

Introduction

The test kit is ***ecting for the quantitative analysis of Aflatoxin M1 in milk, milk powder.

Principle of Test

The Aflatoxin M1 (AFM1) ELISA Test Kit is based on a competitive colorimetric ELISA assay.

The toxin of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin and enzyme-conjugate. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the well. The enzyme-conjugate, will bined with the primary antibody that is complexed to the toxin coated on the plate wells, last, take coloration with TMB substrate. The samples absorbance value is an inverse relationship with AFM1 content. Lastly, co***red with the standard curve can be concluded the AFM1 residues in sample.

Technique Data

Kit sensitivity: 0.02ppb (ng/ml)
reactive mode: 25℃, 30min¡«15min
***ection Limits:
Sample ***ection Limits
Milk 0.04 ppb
Milk powder 0.06 ppb
Cross-reaction rate:
Aflatoxin M1 ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­.100%

Sample Recovery rate:
Milk ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­..¡­¡­. 85¡À15%

Milk powder ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­.. 80¡À15%

Materials required but not supplied

Equipments: microplate reader, printer, mixer or stomacher, nitrogen-drying device, oscillator, centrifuge, measuring pipets, and balance with a reciprocal sensibility of 0.01g;
Micropipettors: single-channel 20 to 200¦Ìl and 100 to1000¦Ìl, and multi-channel 300¦Ìl;
Reagents: acetonitrile, deionized water.
Sample pre-treatment

Instructions
Labware must be clean and the use of disposable pipette tips to avoid contamination of interference results

Solution preparation before sample pre-treatment:
Liquor 1: Sample extracts solution:

84% Acetonitrile-water solution, V (Acetonitrile): V (Deionized water) = V21: V4

Liquor 2: redissolving solution:

2 times dilute the2¡Á concentrated redissolving solution with deionized water to be used for

Contact Us
Company: Nanjing Pars Biochem CO.,Ltd
Contact: Ms. aimee She
Address: mozhoudonglu 12th jiangning
Postcode:
Tel: 86-025-52257530
Fax: 86-025-52257530
E-mail:         


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